动物造模 z-bio 2023-02-14 565

　　Objective: To observe the preventive effect of Ginkgo biloba extract on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's mice of different ages.

　　Methods: 60 C57BL/6 mice at 8 weeks and 32 weeks were randomly divided into negative group, model group, high-dose, medium-dose and low-dose GBE groups and positive group. The PD animal model was established by intraperitoneal injection of MPTP. , Ginkgo biloba extract was used for gavage treatment. Climbing, hanging and swimming experiments were used to detect the motor function of the limbs of the mice, and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and propanediol in the brain tissue of the mice were determined by spectrophotometry. Aldehyde (MDA) content, and immunohistochemical methods were used to observe the changes in the number of tyrosine hydroxylase (TH) and striatal dopamine transporter (DAT) positive cells in the substantia nigra of mice.

　　Results: (1) Compared with the negative control group, the motor function scores, brain GSH-Px activity, TH-positive cells in the substantia nigra and DAT-positive cells in the striatum were significantly decreased in the 8-week-old model group. The MDA content was significantly increased, indicating that the PD model was successfully replicated. Compared with the model group, the motor function scores, SOD and GSH-Px activities, and the average optical density of TH-positive cells in the substantia nigra of the 8-week-old mice in the Ginkgo biloba extract administration group were significantly increased, and the MDA content was significantly decreased. (2) Compared with the negative control group, the motor function scores, the average optical density of TH-positive cells in the substantia nigra and the DAT-positive cells in the striatum of the 32-week-old model group were significantly decreased, the activities of SOD and GSH-Px were decreased, and the brain tissue was significantly decreased. The increase of MDA content indicated that the model was successfully replicated. Compared with the model group, the motor function score, SOD activity, TH in the substantia nigra and the average optical density of DAT-positive cells in the striatum of the 32-week-old mice in the Ginkgo biloba extract administration group were significantly increased, and the content of MDA in the brain tissue was significantly decreased.

　　Conclusion: Ginkgo biloba extract has a certain preventive effect on MPTP-induced PD mice of different ages.