动物造模,2型糖尿病 z-bio 2022-07-25 251

　　OBJECTIVE: To investigate the possible link between the alcoholic extract of coreopsis bicolor, intestinal flora and type 2 diabetes mellitus (T2DM).

　　Methods: The model group (A) was collected, the high-dose coreopsis ethanol extract group was 1.8 g/kg (B), the two-color coreopsis alcohol extract medium-dose group was 1.2 g/kg (C), and the metformin control group was 0.2 g. /kg (D), blank control group (E) stool samples, 16S rDNA real-time fluorescence quantitative PCR technology was used to detect the levels of Clostridium sphaeroides and Bacteroides polymorpha in stool samples; Pearson analysis method was used to compare the level of target bacteria and each species. Correlation analysis was performed on the fasting blood glucose (FBG) of the mice in the group.

　　Results: ①Compared with the normal group, the levels of Clostridium sphaeroides and Bacteroides polymorpha in the model group were increased (P=0.017, P=0.002). ②Compared with the model group, the levels of Clostridium sphaericus and Bacteroides polymorpha in the two-color coreopsis alcohol extract high-dose group decreased, and there was a statistical difference (2 weeks: P=0.027, P=0.006; 4 weeks: P =0.007,P=0.012). ③The level of Clostridium sphaericus and Bacteroides polymorpha were positively correlated with the level of FBG.

　　Conclusion: The two-color coreopsis alcohol extract has a certain effect on the intestinal flora of mice with type 2 diabetes, and there is a correlation with its blood sugar level.