动物造模 z-bio 2022-07-26 300

　　Objective: To investigate the morphological changes of adipocytes in different periods of cold acclimation, and to provide a morphological basis for whether cold acclimation can induce the production of beige adipocytes in white adipose tissue.

　　Methods: The sections were compared by hematoxylin-eosin staining, immunohistochemical staining and immunofluorescence staining.

　　Results: With the prolongation of cold acclimation time, the morphology of both white adipocytes and brown adipocytes became significantly smaller, and the expression of uncoupling protein 1 (UCP1) in brown adipose tissue increased. Browning has occurred, resulting in some light brown adipocytes with UCP1 expression, i.e. beige adipocytes.

　　Conclusion: Different stained sections can not only clearly observe the morphological changes of adipocytes, but also observe the changes of UCP1 expression from the antibody-positive color, which provides basic materials for the study of adipose tissue of Chinese shrew.