动物造模 z-bio 2022-07-26 344

　　Objective: To investigate the optimal anticoagulation ratio of rats, and to analyze the influence of insufficient blood volume on the four test results of coagulation.

　　METHODS: Sixty rats were divided into two groups, and abdominal aortic blood was collected by vacuum blood sampling method for 12 hours on an empty stomach. The first group of 20 were used for whole blood cell determination. An automatic blood cell counter was used to detect the hematocrit (hematocrit, HCT) and platelet count (platelet, PLT). The second group of 40 rats, 2 tubes of blood were drawn from each rat, were divided into 1:9 (control group) and 1:5 ( Experimental group), 1:8 (experimental group) and 1:7 (experimental group), centrifuged to obtain platelet-deficient plasma. Automatic blood coagulation analyzer detects four coagulation items: prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (Fibrinogen, FIB).

　　RESULTS: The HCT (%) of SD rats was 41.7 ± 2.9, and the PLT (×109/L) was 1114 ± 173. With the increase of anticoagulation ratio, PT, APTT, TT prolonged, FIB decreased. Compared with the control group: 1:8 experimental group, the difference was not statistically significant. In the 1:7 experimental group, there was no significant difference in the other three indexes except TT. 1:5 experimental group, the difference was statistically significant.

　　Conclusion: The results of the four coagulation items are affected by the anticoagulation ratio. 1:9 was the best anticoagulation ratio for rats, and 1:8 was acceptable. Rats have their unique physiological characteristics. It provides basic scientific data and basic theoretical basis for the related research on rats.