动物造模 z-bio 2022-11-16 576

　　Objective: To compare the effects of two positive substances, human serum albumin and ovalbumin, on active systemic allergy in guinea pigs, so as to provide a better positive control for allergy tests.

　　Methods: The guinea pigs were randomly divided into 14 groups, with human serum albumin, ovalbumin (2, 10, 100 mg/pig), 0.9% sodium chloride injection and other test substances as controls, to study different sensitizing doses, challenge Under the conditions of dose and challenge time, the reaction of guinea pig systemic active allergy.

　　RESULTS: Within the dose range of 2-100 mg/dose, the incidence of active systemic allergic reactions in human serum albumin and ovalbumin guinea pigs was 100%. Within the dose range of 2-10 mg/dose, the degree of allergic symptoms increased with the increase of the sensitizing dose and the provocative dose, and the same dose of ovalbumin had a stronger degree of reaction than human serum albumin.

　　Conclusion: In the active systemic allergy test of guinea pigs, the positive control is recommended to use ovalbumin at a dose of 2 mg/guinea pig.