动物造模,慢性肾病大鼠模型 z-bio 2022-08-05 433

　　Objective To observe the expression of HIF-1α, RhoA and SOX9 in adenine-induced chronic kidney disease model in rats and their relationship with renal interstitial fibrosis.

　　Methods Twenty rats were randomly divided into control group (Con group) and model group (adenine-induced CKD rat model). Rats were sacrificed at 3 and 6 weeks, respectively. Observe the morphological changes of the kidneys; detect the renal function and 24-hour urine protein levels of the rats; HE staining to observe the renal pathological changes; Sirius red staining to observe the status of tubulointerstitial fibers; immunohistochemical staining to observe the HIF-1α, RhoA, Expression of SOX9, Col-I and α-SMA proteins; RT-PCR detection of HIF-1α, RhoA and SOX9 and Col-I, α-SMA mRNA levels in rat kidneys, analysis of the relationship between the expression levels of HIF-1α, RhoA and SOX9 in kidney The correlation of tubulointerstitial fibrosis; the serum SOX9 levels of the two groups of rats were detected by ELISA.

　　Results Compared with the Con group, the 24-hour urine protein, serum BUN and Scr of the rats in the CKD group were significantly increased (P<0.05). The expression of HIF-1α, RhoA, SOX9 protein and mRNA in the CKD group were increased by immunohistochemistry and RT-PCR; the serum SOX9 level in the CKD group was higher than that in the control group, and increased with the modeling time. extended increase. Correlation analysis showed that HIF-1α, RhoA, SOX9 mRNA expression was correlated with Col-I and α-SMA, and there was also a positive correlation among HIF-1α, RhoA, and SOX9.

　　Conclusion In adenine-induced CKD rat model, there are high expressions of HIF-1α, RhoA and SOX9, which may be closely related to the process of renal interstitial fibrosis; the level of serum SOX9 is expected to be a molecular marker for early diagnosis of CKD.