动物造模,白血病小鼠模型 z-bio 2023-03-04 450

　　Objective To investigate the effect of silencing the expression of heme oxygenase-1 on leukemia in a mouse model of acute monocytic leukemia.

　　Methods U937 cells were transfected with lentivirus to silence the expression of HO-1. U937 cells of different treatment groups were injected subcutaneously into the armpits of NOD/SCID mice to establish a model, which was divided into blank group and experimental group (U937 group, silent HO group) -1 group, empty vector group). After the identification model was successfully constructed, the tumor formation time, tumor size, tumor infiltration to surrounding tissues, peripheral blood leukocyte and platelet counts, hemoglobin content and survival time were compared in different groups of mice.

　　Results Fluorescence microscopy and Western blot confirmed that the lentiviral transfection was successful. Compared with the blank group, the mice in the experimental group formed a mass in the armpit, the white blood cell count in peripheral blood was significantly increased, leukemia cells were seen on the smear, and a group of cells were found to express CD13, CD14, and CD64 in the bone marrow fluid, confirming that the modeling was successful. Compared with the U937 and empty vector groups, the time required for the appearance of subcutaneous mass in the M5 mouse model in the silenced HO-1 group was significantly longer (P<0.05), the growth rate of the mass was slower (P<0.05), and the peripheral blood The increase of white blood cells and the decrease of platelets were slower (P<0.05), and the survival time was significantly prolonged (P<0.05).

　　Conclusion Silencing the expression of HO-1 can slow down the progression of leukemia in the M5 mouse model, and HO-1 is expected to become one of the therapeutic targets of M5.