动物造模 z-bio 2022-08-05 378

　　Objective To compare the differences between the sodium laurate injury endothelium method and the microsphere embolization method to establish a rat model of coronary microcirculation disorder, and to explore a stable animal model that can be used for disease pathogenesis and drug research.

　　Methods Thirty male SD rats were randomly divided into sham operation group, sodium laurate group and microsphere embolization group, 10 rats in each group. After anesthesia, the rats were thoracotomy, and 0.2 mL of sodium laurate (1 g/L) or embolization microspheres (40-120 μm, 1000) were injected into the left ventricle, and the sham operation group was injected with an equal volume of normal saline. After 24 h, the animals were anesthetized again to measure the relevant indicators, and then the animals were sacrificed.

　　Results In the sodium laurate group, coronary microvascular thrombosis and myocardial cell injury were observed, and there were no differences in cardiac function, hemodynamics and myocardial enzymes compared with those in the sham operation. In the microsphere embolization group, the microspheres were embolized in coronary microvessels and myocardial injury, the left ventricular ejection fraction and the left ventricular shortening rate were significantly reduced, the maximum increase rate of left ventricular pressure was significantly reduced, the creatine kinase MB isoenzyme and the lactate dehydrogenase were significantly reduced. activity increased significantly.

　　Conclusion Both modeling methods can cause coronary microcirculation disorder, and have a certain impact on the cardiac function of rats. Compared with the sodium laurate method, the key indicators of the animal model established by the microsphere embolization method are more stable, and can be widely used in the study of the pathogenesis and drug treatment of coronary microcirculation disorders.