动物造模,严重失血性休克模型 z-bio 2022-08-05 541

　　Objective The current animal models of hemorrhage often show a large range of blood pressure fluctuations and limited brain tissue damage. Therefore, this study attempted to establish a long-term severe hemorrhagic shock model with no obvious blood pressure fluctuations in rats.

　　Methods The empty 10 mL syringe barrel was fixed at a position of 47.58 cm above the surgical board as a blood reservoir. After anesthesia, the rats were cannulated through the right femoral artery, and the blood was automatically released to the blood bank by using the pressure difference to induce shock. Mean arterial blood pressure (MAP) was controlled by slightly moving the syringe barrel up and down during shock to adjust blood levels in the syringe. Twenty-eight male SD rats were randomly divided into 4 groups according to different resuscitation methods. The shock in each group lasted for 3 hours. During the experiment, the physiological conditions and hemodynamic indexes of the rats in each group were monitored in real time, and blood was collected before and after shock for arterial blood gas analysis. Analysis, HE staining was used to observe the damage of hippocampal neurons in the brain.

　　Results During the 3-h hemorrhagic shock, the MAP in each group could be accurately maintained within the range of 31-35 mmHg; at the end of the 3-h shock, all the rats were able to survive, and 6 of the 7 rats in the non-resuscitation (RN) group The patients died within 2 hours after the shock, and the resuscitation (RB and RR) groups could survive within 3 hours after the shock. The maximum blood loss occurred 20-30 minutes after sexual shock, and the maximum blood loss and the final blood loss were very similar; the pathological results showed that all hippocampal neurons were significantly damaged.

　　Conclusion The technique of blood storage in the empty barrel of a syringe can accurately control MAP, prevent obvious blood pressure fluctuations, and help to establish a model of persistent severe hemorrhagic shock. This hemorrhagic shock model can induce reliable brain injury, is highly reproducible, and can be used for both constant pressure and volume, so it can be used to study the pathophysiological changes and therapeutic effects of hemorrhagic shock.