动物造模,原位移植瘤动物模型 z-bio 2023-02-27 687

　　Objective To establish an animal model of neuroblastoma adrenal orthotopic transplantation, and to explore its advantages over the traditional subcutaneous ectopic tumor transplantation model.

　　Methods Human neuroblastoma cells were cultured in vitro and inoculated into the left periadrenal fat of SCID-Beige mice. The tumor tissue was harvested on the 14th, 21st, and 28th day after tumor cell inoculation, and transplanted with traditional subcutaneous ectopic transplantation. The tumor formation rate, tumor growth rate and histopathological indicators were compared.

　　Results After injection of the same number of tumor cells, the tumor formation rate of orthotopic transplantation mice was 100% at the three sampling time points, while the tumor formation rates of subcutaneous heterotopic transplantation mice were 33%, 67%, and 78% at the three sampling time points, respectively. %, the tumor formation rate of orthotopic transplanted tumor was higher than that of subcutaneous heterotopic transplanted tumor. On the 14th, 21st, and 28th days of inoculation with tumor cells, the tumor volumes of orthotopic transplanted tumors were (116.21±78.82), (245.32±97.31), (3091.21±2042.39) mm3, respectively. The tumor volume of the subcutaneous heterotopic transplanted tumor was (7.26±6.04), (41.67±38.52), (292.14±167.12) mm3. Compared with the subcutaneous heterotopic transplanted tumor, the original The transplanted tumor grows faster and the seed tumor volume is larger. The HE staining of tumor tissue indicated that the orthotopic xenograft tumor tissue had a higher density of tumor cells than the subcutaneous ectopic xenograft tumor.

　　Conclusion The animal model of neuroblastoma adrenal orthotopic transplantation is feasible in technology. Compared with the subcutaneous ectopic transplantation tumor model, it has the advantages of higher tumor formation rate and faster tumor growth rate; it is more suitable for clinical neuroblastoma than the subcutaneous ectopic transplantation tumor. Growth location, providing a more accurate and efficient animal model for the in-depth study of neuroblastoma.