动物造模,肥胖型多囊卵巢综合征伴胰岛素抵抗 z-bio 2022-08-08 401

　　Objective To establish an animal model of obese polycystic ovary syndrome with insulin resistance in two different ways combined with high-fat diet, and to compare the changes in endocrine and metabolic characteristics.

　　Methods The rats were randomly divided into 3 groups: control group, given normal chow and purified water; Letrozole group, given intragastric administration of letrozole (1 mg/kg); HCG+INS group, given subcutaneous injection of chorionic gonadotropin and insulin ( HCG+INS), both groups were fed with high-fat diet and 5% glucose solution. Gram staining was used to observe the changes of the two estrous cycles; on the 23rd and 30th days, the wet weight of the uterus and ovary was measured; hematoxylin-eosin (HE) staining was used to observe the histopathological changes of the ovary; fasting blood glucose (FBG) was measured by blood biochemical analyzer ), blood lipids (triglyceride (TG) and total cholesterol (TC) changes; enzyme-linked immunosorbent assay (ELISA) assay for sex hormones (follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), total Changes in the levels of testosterone (T), serum inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β); calculation of insulin resistance index HOMA-IR; determination of ovarian TNF-α by real-time fluorescence quantitative PCR and IL-1β mRNA changes.

　　Results Compared with the control group, both estrous cycles in the Letrozole group and the HCG+INS group were disordered, but the HCG+INS group had occasional ovulation; on the 23rd day, the wet weight of the uterus and the level of E2 in the Letrozole group were significantly reduced, and the wet weight of the ovary and the ovary were significantly decreased. The levels of INS, HOMA-IR, TG and TC were significantly increased, and the levels of INS, HOMA-IR, TC, TG and LH were significantly increased, and the level of E2 was significantly decreased in the HCG+INS group (P<0.05, P<0.01). On the 30th day, the levels of FBG, INS, HOMA-IR, TG, FSH, LH and serum TNF-α and IL-1β levels and ovarian mRNA were significantly increased in the two groups. , ovarian polycystic changes and other characteristics are more obvious.

　　Conclusion Both modeling methods can successfully induce PCOS-IR animal model, and the changes in body weight, endocrine and metabolic indexes of letrozole modeling method on the 30th day are more in line with the pathological characteristics of obese PCOS-IR.