动物造模 z-bio 2023-02-27 475

　　Objective To optimize the detection method of Pasteurella pneumophila in mice, improve the detection sensitivity to strengthen the daily monitoring of Pasteurella pneumophila, and explore the feasibility of drug purification in infected mice.

　　Methods The direct plating method and the modified enrichment method were used to detect Pasteurella pneumophila at the same time, and the detection effects of the two methods were compared. The susceptibility of locally isolated Pasteurella pneumophila strains to 12 antibiotics was investigated using the disc diffusion method. Forty-eight female C57BL/6 mice were randomly divided into 6 groups, with 8 mice in each group. Enrofloxacin was administered with different concentrations of enrofloxacin for 6 consecutive weeks to evaluate the safe dosage of enrofloxacin in C57BL/6 mice. Mice were infected with locally isolated Pasteurella pneumophila, and then the mice positive for Pasteurella pneumophila were given drinking water containing different concentrations of enrofloxacin to test the effect of enrofloxacin on Pasteurella pneumophila infection The clearance effect in mice determines the drug purification scheme.

　　Results The detection rates of Pasteurella pneumophila by direct plating method and modified enrichment method were 1.3% and 15.1%, respectively (P＜0.05). Sensitive to antibiotics such as cefdrozole; Enrofloxacin at a dose below 300 mg·kg-1·d-1 has no obvious toxic and side effects in mice; a dose above 85 mg·kg-1·d-1 Enrofloxacin Floxacin was administered to the drinking water of Pasteurella pneumophila-positive adult mice, and all of them turned negative after 2 weeks of treatment, but did not turn positive after 6 weeks of drug withdrawal.

　　Conclusion The sensitivity of the improved enrichment method for the detection of Pasteurella pneumophila is higher than that of the direct plating method, and the process is simple and convenient, which is suitable for daily health monitoring in animal rooms. The administration of enrofloxacin in drinking water at a dose of 85 mg·kg-1·d-1 or more can safely and effectively eliminate Pasteurella pneumophila in mice, and can be used as an emergency purification for mice infected with Pasteurella pneumophila if necessary. Program.