动物造模,肉芽肿小叶性乳腺炎大鼠模型 z-bio 2022-08-08 371

　　Objective To establish an animal model of granulomatous lobular mastitis by using the homogenate of human granulomatous lobular mastitis and the suspension of complete Freund's adjuvant.

　　Methods Twenty-five SD rats with a history of pregnancy and childbirth were randomly divided into 5 groups: blank control group, complete Freund's adjuvant group, normal breast tissue homogenate group, diseased breast tissue homogenate low-dose group, and diseased breast tissue homogenate high-dose group. Dosage group, 5 in each group. diseased breast tissue

　　In the homogenate group, complete Freund's adjuvant suspension prepared from human granulomatous mastitis tissue homogenate was inoculated into the 3rd and 4th pair of mammary gland tissues of rats (injection of 0.2 mL in the high-dose group and 0.1 mL in the low-dose group) The normal breast tissue homogenate group was inoculated with normal breast tissue homogenate from patients with granulomatous mastitis; the blank control group was inoculated with normal saline (ie 0.9% NaCl solution); the complete Freund's adjuvant group was inoculated with complete Freund's adjuvant. After the model was established, the rats were kept for 14 days, and the histomorphology and histopathological characteristics after HE staining were observed, and the effect of establishing the model of granulomatous lobular mastitis was compared.

　　Results Compared with the blank control group, the complete Freund's adjuvant group and the normal breast tissue homogenate group, the high and low doses of the lesion breast tissue homogenate group showed different degrees of morphological changes and pathological characteristics unique to granulomatous lobular mastitis. The difference between groups was statistically significant (P<0.001).

　　Conclusion The homogenate of human granulomatous lobular mastitis specimens and the suspension of complete Freund's adjuvant can successfully establish a rat model of granulomatous lobular mastitis, which lays a foundation for the etiology of the disease.