动物造模,糖尿病肾病模型 z-bio 2022-08-08 587

　　Objective To compare and study the stability of C57BL/6J mice model of diabetic nephropathy induced by different doses of streptozotocin combined with high-sugar and high-fat diet.

　　Methods Forty-eight male C57BL/6J mice were randomly divided into: control group (injected with equal volume of citrate buffer, 12 mice), single high-dose (150 mg/kg STZ, single intraperitoneal injection combined with high-fat and high-glucose, 18 only), multiple small doses (50 mg/kg STZ, continuous injection for 4 days combined with high-fat and high-sugar, 18 rats). Changes in fasting blood glucose (FBG), water intake, blood lipids, 24-hour urine protein, renal function and renal pathology were detected by the two modeling methods.

　　Results After the injection of STZ, the weight of the mice in the single high-dose group increased slowly, while the weight of the mice in the multiple low-dose group showed a negative increase; the FBG and water intake of the single high-dose mice reached the peak 4 weeks after the injection of STZ, and the 24-hour urine protein was at The peak value was reached after 6 weeks of STZ injection, and then gradually decreased; FBG, 24h urine protein and water intake in the multiple low-dose group increased steadily and maintained at a high level; at the end of the experiment, the FBG, 24h urine protein, 24h urine protein and water intake of the mice in the multiple low-dose group were Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), serum creatinine (Scr), serum urea nitrogen (BUN) , kidney index (kidney index, KI) and glomerular reducing sugar levels were significantly higher than the single high-dose group (P<0.05), and the renal pathological changes were more obvious.

　　Conclusion Multiple low-dose injections of STZ combined with high-sugar and high-fat diet can establish diabetic nephropathy in mice with high model rate, low mortality and good stability. It has similar pathogenesis and pathological characteristics of human diabetic nephropathy, and is an ideal model for diabetic nephropathy. method.