动物造模 z-bio 2022-08-11 344

　　Objective To establish a mouse model of oligodendrocyte-specific knockout of fibroblast growth factor 9 (FGF9), and to further study the role of FGF9 in neural development.

　　Methods Olig1-Cre transgenic mice were crossed with FGF9 transgenic mice (FGF9flox / flox ), female FGF9flox / wt / Olig1-Cre+ and male FGF9flox / flox were selected for co-cage mating, and the F3 generation obtained oligodendrocyte-specific knockout. FGF9 gene mice (FGF9flox / flox / Olig1-Cre+ ). In order to confirm the specificity and effectiveness of conditional gene knockout, the genomic DNA of mouse tail tissue was extracted, its genotype was identified by PCR technology, the expression of FGF9 protein was verified by protein electrophoresis and confocal laser, and its phenotype was observed.

　　Results The FGF9flox/flox/Olig1-Cre+ mice were successfully constructed from the gene and protein levels. Preliminary phenotypic analysis showed that the knockout mice were viable and fertile, with the same survival period as the control group, but with slow development and significant weight loss.

　　Conclusion The mice with specific knockout of FGF9 gene in oligodendrocytes were successfully obtained, and the conditional knockout of FGF9 gene caused slow development of mice.