动物造模,人脑胶质瘤大鼠移植模型 z-bio 2022-08-15 501

　　Objective To initially establish a rat xenograft model of human glioma by using immunosuppressive agents to provide an ideal experimental tool for clinical and basic research.

　　Methods The commonly used immunosuppressants rapamycin and cyclosporin A (cyclosporin (A) were selected and given to SD rats by gavage 3 days before surgery. group, cyclosporine A single drug (CsA) group, combined drug (Rapa+CsA) group and control group. Human glioma U87-MG cells were used for SD rat brain orthotopic transplantation and right back subcutaneous After transplantation, the changes of tumor volume and body weight in rats were monitored. When the tumor grew to about 60o mm3, the growth of in situ glioma in rats was detected by near-infrared fluorescence in vivo imaging; Tumor histological morphology was detected by HE staining and immunohistochemistry.

　　Results Compared with the Rapa group and the CsA group, the combined drug (Rapa + CsA) showed obvious advantages in immunosuppression, and the model success rate reached 100% (the success rate of the Rapa and CsA single drugs were 0% and 40%, respectively) . The body weight of the rats in the combination group was significantly smaller than that in the Rapa group, the CsA group and the control group. In vivo imaging found that the near-infrared fluorescent dye was enriched in the rat brain, and the corresponding histopathological analysis determined that it was a glioma. Immunohistochemical staining with human mitochondrial Mitochondria antibody showed strong positivity at the tumor site.

　　Conclusions The combination of drugs (Rapa+CsA) for immunosuppression and inoculation of human glioma U87-MG cells can successfully establish a rat xenograft model of human glioma. Pathological analysis confirms the histological morphology of human glioma.