动物造模,溃疡性结肠炎模型 z-bio 2022-08-15 349

　　Objective To study the formation of ulcerative colitis in mice after gavage or free drinking of dextran sodium sulfate.

　　Methods Thirty-two male C57BL/6 mice were selected and randomly divided into blank group, 3% DSS free-drinking group, 5 g/kg and 6 g/kg DSS gavage groups, 8 mice in each group, and administered continuously for 7 days. The water intake, body weight, fecal characteristics and blood consumption of the mice in each group were recorded every day, and the disease activity index (DAI) score of the mice was carried out. On the 8th day, the mice were sacrificed, and the colon, thymus and spleen of the mice were isolated, the thymus and spleen were weighed, and the immune organ index was calculated; the length of the colon was measured, the pathological changes of the colon were observed, and histopathological scoring was performed.

　　RESULTS: The total DSS intake of mice in each administration group on 7 days was more than 30 mg/g. Compared with the blank group, there was no significant difference in the DAI score of the mice in the gavage group (P>0.05), and the DAI score of the mice in the white drinking group increased significantly from the 3rd day (P<0.01). Compared with the blank group, the colons of the mice in each administration group were significantly shortened (P<0.01), but="" the="" difference="" in="" thymus="" index="" was="" not="" statistically="" significant="" p="">0.05). The gastric group was more obvious (P<0.05). In terms of histopathological changes, the colon of mice in the 6 g/kg gavage group had slight pathological changes; the pathological changes in the colon of the mice in the free drinking group were more significant, and the histopathological scores were significantly higher than those in the gavage groups (P <0.01 or P<0.05).

　　Conclusion When using DSS to establish a mouse ulcerative colitis model, the free drinking method is more effective than the gavage model.