动物实验,造血干细胞 z-bo 2020-08-20 641

　　Radiation injects hematopoietic stem cells (HSC) intravenously into radiation-immunized mice. HSC needs to consume T cells to reduce the graft-versus-host immune response. Successful HSC transplantation can rebuild the human immune hematopoietic system in mice. The operation steps are as follows.

　　Put 12-week-old NOG or NSG mice into a sterile container equipped with a filter. The mice were irradiated with 240 cGy radiation (137 Cs radiation source). Human HSC is injected within 4-24 hours. The irradiated mice can kill mouse HSCs and provide instructions for transplantation of injected human HSCs. Prepare human umbilical cord blood cells and use CD3 antibody magnetic beads to bind and remove CD3 + T cells. 0.5 ml of HSC cells in PBS buffer containing 3x10000/CD34 + were injected through the tail vein of mice. Human HSCs are maintained in mice for 10-12 weeks. At the same time, human HSC cells gradually grow and differentiate to produce human T and B cells. Human CD45+ cells can be detected 4 weeks after HSC injection, which indicates that the human immune system has been successfully rebuilt in mice.