动物造模,慢性高尿酸血症肾损害大鼠模型 z-bio 2022-08-17 336

　　Objective To provide a model tool for drug development against chronic hyperuricemia renal damage by establishing a rat model of chronic hyperuricemia renal damage.

　　Methods Forty male SD rats were randomly divided into 5 groups: normal group, group A (2% potassium oxonate + 12% yeast extract + 86% normal diet), group B (0.15% adenine + 10% yeast extract + 89.85% common feed), group C (100 mg/kg adenine + 1500 mg/kg potassium oxonate, once a day in the morning and evening) and group D (50 mg/kg kg adenine + 1500 mg/kg potassium oxonate, gavage once a day in the morning). The observation time was 5 weeks. The body weight of rats in each group, serum uric acid, creatinine, blood urea nitrogen and other indicators were detected every week.

　　Results Compared with the normal group, the rats in group C lost weight, the ratio of kidney weight to body weight increased, serum uric acid, creatinine and blood urea nitrogen were all increased, and the color of the kidneys changed significantly. degree of damage.

　　Conclusion 100 mg/kg adenine + 1500 mg/kg potassium oxonate is the best way to establish a rat model of chronic hyperuricemia with renal damage by intragastric administration in the morning and evening.