动物造模,慢性血栓小鼠模型 z-bio 2023-03-16 580

　　Objective To screen the conditions of chronic thrombosis mouse model by treating mice with different concentrations of carrageenan solution by continuous intraperitoneal injection.

　　Methods Seventy ICR male mice were randomly divided into 7 groups with 10 mice in each group, namely: blank control group, 0.02% dose group, 0.04% dose group, 0.06% dose group, 0.08% dose group, and 0.10% dose group. , 0.20% dose group. The mice in the blank group were intraperitoneally injected with normal saline (0.01 mL/g), and the other groups were intraperitoneally injected with the corresponding concentration of carrageenan solution (0.01 mL/g), once a day; the mice in each group were reared at (20±1) ℃ room temperature and free access to food and water. The occurrence of black tail (black tail rate and length of black tail) in each group was observed every day; sampling was performed immediately after embolization; blood was collected to detect four items of blood coagulation, and oxidative stress indexes and inflammatory factor indexes in colon tissue and plasma were detected.

　　Results The 0.06% dose group had 2 emboli on the 5th day and 8 emboli on the 6th day; compared with the blank control group, the FIB content of the 0.06% dose group was significantly increased (P<0.05), and the APTT was significantly shortened (P<0.05), TT was significantly prolonged (P<0.05), and PT was significantly prolonged (P<0.01); SOD, GSH-Px in colon tissue and plasma in 0.06% dose group were significantly decreased (P<0.01), MDA significantly increased (P<0.05); TNF-α, IIIL-1β in colon tissue and plasma in 0.06% dose group were significantly increased (P<0.05), and IL-10 was significantly decreased (P<0.05).

　　Conclusion It is preliminarily concluded that continuous intraperitoneal injection of 0.06% carrageenan solution (0.01 mL/g) for 6 days (once a day) at room temperature (20±1) ℃ is the optimal condition for establishing a chronic thrombosis mouse model .