动物造模,急性肺损伤大鼠模型 z-bio 2022-08-17 361

　　Objective To establish a rat model of acute lung injury by tracheal instillation of lipopolysaccharide, and to observe the changes of lung injury in different periods.

　　Methods Thirty-two healthy SD rats were randomly divided into normal group and model group, with 8 rats in normal group and 24 rats in model group. The model group was divided into three subgroups according to the different duration of LPS instillation; 3 h group, 6 h group and 12 h group, with 8 mice in each group. The AII rat model was established by exposed tracheal instillation of LPS (2 mg/kg). Through the general condition of the rat, the general observation of the lung, the detection of lung function, the calculation of the ratio of wet to dry weight of the lung, the interleukin-1β (IL-1β) and interleukin-8 (IL-8) in the bronchoalveolar lavage fluid (BALF) ), tumor necrosis factor-α (TNF-α) detection and malondialdehyde (MDA), superoxide dismutase (SOD) detection in lung tissue, hematoxylin-eosin staining (HE staining) histomorphological observation, the evaluation of different Changes in acute lung injury over time.

　　Results After modeling, the survival rate of rats in the model group was 100%. The general condition of the rats showed that, compared with the normal group, the 3 h group was in a state of indifference, decreased food intake, significantly decreased activity, increased mucus secretions in the nasal cavity, increased breathing rate, and could hear and wheeze. The gross observation of the lungs showed that in the 3 h group, liver-like changes of the lung tissue were seen in the left and right hilum of the lungs, blood spots were scattered on the left and right lung lobes, and the bleeding sites were bright red. Pulmonary function showed the forced expiratory volume in 0.1 second (FEV 0.1), the forced expiratory volume in 0.3 second (FEV 0.3) and the ratio of each occupied forced vital capacity (FVC) (FEV0.1/FVC; FEV0. 3/FVC) were significantly decreased (P<0.05.P<0.01); the ratio of lung wet to dry weight was significantly increased (P<0.01); the contents of IL-1β, IL-8 and TNF-α in BALF were significantly increased (P<0.01). < 0.01), the content of MDA was significantly increased (P < 0.01), and the content of SOD was significantly decreased (P < 0.01); HE staining showed obvious thickening of alveolar septa, pulmonary interstitial edema, and erythrocyte exudation.

　　Conclusion Exposure tracheal instillation of LPS can cause significant decrease in lung function, severe pulmonary inflammation, oxidative-antioxidant imbalance, severe pulmonary edema and pulmonary hemorrhage in rats, leading to acute lung injury, which is more conducive to ALI in the 3-hour period. Construction of the rat model.