动物造模,盐敏感性高血压 z-bio 2022-08-23 305

　　Objective To establish a mouse model of salt-sensitive hypertension, and use the antihypertensive drug nifedipine to verify the validity of the model.

　　Methods C57BL/6J mice were used as the research objects, and the normal group fed with conventional feed, the high-salt group fed with high-salt feed, and the intervention group fed with nifedipine after high-salt feed were set respectively. The blood pressure changes of the mice in each group were measured by a non-invasive blood pressure measurement system; the biochemical indexes such as liver function, renal function, blood lipid, blood sugar and ions in the serum of the mice were measured by a blood biochemical analyzer; Histological changes in rat liver, kidney and carotid artery.

　　Results Compared with the blood pressure of the normal group, the systolic and diastolic blood pressure of the high-salt group were significantly increased (both P<0.01), while the blood pressure of the intervention group was significantly decreased (both P<0.01). Compared with the normal group, the levels of liver function indexes aspartate aminotransferase, alkaline phosphatase, albumin, serum total protein and direct bilirubin, as well as blood lipid indexes cholesterol, renal function indexes uric acid and calcium in the high-salt group mice There were significant changes in ion and magnesium ion levels (both P<0.05), while the intervention group recovered gradually. Histopathology showed that the liver and kidney of the mice in the high-salt group were damaged to varying degrees.

　　Conclusion High-salt diet can successfully establish a salt-sensitive hypertension mouse model, and nifedipine can effectively reduce blood pressure in the model mice.