动物造模,肥胖模型 z-bio 2022-08-23 278

　　Objective To observe the effect of using high-calorie diet to establish an obesity model in rats, as well as the modeling differences between the two breeds of rats, and to compare different methods of evaluating obesity and screening methods of obesity-sensitive animals, so as to provide guidance for the preparation of animal models when studying the function of weight-loss products. refer to.

　　Methods A total of 120 SD and Wistar male rats were selected and randomly divided into 2 groups (blank control group and model group) according to body weight, with 60 rats in each group. The blank control group was given maintenance feed, and the model group was given high-calorie feed with improved formula. The body weight and body length of the rats were recorded once a week, the feed quality was weighed twice a week, and the Lee's index, food intake and food utilization rate were calculated. 6 weeks. After 2 or 6 weeks of feeding, 30 rats in each group were selected to detect blood biochemical indexes. After autopsy, bilateral peri-renal fat and peri-testicular fat were collected and weighed, and the fat/body ratio was calculated. The correlation between body weight gain, Lee's index and obesity and obesity sensitivity in rats was analyzed, and the body weight gain and obesity were compared between the rats whose body weight gain was in the bottom 1/3, 10%, and 20% respectively after feeding for 2 weeks. Correlation.

　　Results Compared with the blank control group, the body weight, Lee's index, body weight, food utilization rate, body fat mass and fat/body ratio of the model groups of the two strains increased, food intake decreased, serum glucose and triacylglycerol increased. Levels increased, and high-density lipoprotein cholesterol levels decreased (both P<0.05). comparing="" sd="" rats="" and="" wistar="" there="" was="" no="" significant="" difference="" in="" each="" index="" of="" the="" model="" group="" p="">0.05), while the body length, Lee's index and food utilization rate of the blank control group were occasionally different (all P<0.05). The serum high-density lipoprotein cholesterol levels of rats in the blank control group were significantly different when fed for 2 weeks and 6 weeks (P<0.05). The correlation between weight gain and obesity and obesity sensitivity of the two breeds of rats was significantly greater than that of Lee's index and obesity and obesity sensitivity (both P<0.05). The correlation between body weight gain and obesity in rats was significantly better than that in rats whose body weight gain was ranked in the bottom 1/3, and the correlation coefficient of 10% was the highest.

　　Conclusion The obesity model of SD and Wistar rats can be successfully established with the improved high-calorie diet, and there is no significant difference between the two breeds; body weight gain is better than Lee's index as an indicator for obesity evaluation and obesity-sensitive animal screening, and for screening obesity-sensitive animals The method of excluding the bottom 10% of body mass gain can be selected.