动物造模,肝外型胆汁淤积模型 z-bio 2023-03-24 365

　　Objective To evaluate the advantages and disadvantages of three surgical methods for extrahepatic cholestasis model in rats using serology, imaging and pathology.

　　Methods Fifteen female SD rats were randomly divided into traditional group, modified group and electrocautery group. Cholestasis was modeled by frequency electrocautery coagulation method (n = 5), CT scan and body weight were measured before and after operation to compare liver density and bile duct diameter; 7 days after operation, three groups of rats were collected blood and measured. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), direct bilirubin (DBIL); rats were killed by anesthesia overdose after blood collection, and part of the liver tissue was taken from 4.2% to 5% . 2% formalin solution fixed, paraffin section HE staining observed pathological changes.

　　Results The rats in the three groups had different degrees of liver injury and cholestasis 7 days after modeling. The traditional group had higher AST, TBIL and DBIL than the electrosurgical group (P<0.05), and the traditional group had higher AST, ALT, TBIL and DBIL than the modified group (P<0. dbil="" p="">0. 05 ); pathology showed that the degree of bile duct hyperplasia and bile duct wall thickening in the traditional group was the most serious among the three groups, the degree of the modified group was the least, and the electrocautery group was between the two; Electrosurgery group (P<0. p="">0. 05); traditional group liver CT value was significantly lower than that before operation (P<0. ct="" p="">0. 05).

　　Conclusion Both the electrocautery method and the improved method are more effective in prolonging the course of cholestasis in rats, and can better simulate some human extrahepatic cholestasis diseases, which provides the possibility for dynamic observation of the pathological process of cholestasis.