动物造模,帕金森模型 z-bio 2023-04-03 481

　　Objective To establish a stable 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's model in C57BL/6 mice, and to evaluate the model's behavior.

　　Methods The administration groups were established, and the administration doses were 35 mg/kg, 30 mg/kg, 25 mg/kg, and the control group was given 30 mg/kg normal saline. The tremor palsy score, open field test, pole climbing test, suspension test and swimming test were performed respectively, and comprehensive statistics were carried out. The differences between the administration group and the control group and between each administration group were analyzed. At the same time, HE and immunohistochemical staining with TH antibody were performed to observe the changes of neurons and nerve fibers in the substantia nigra and striatum.

　　Results Compared with the control group, the scores of the administration groups were significantly increased, but the behavioral scores of the 25 mg/kg dose group were significantly lower than those of the 35 mg/kg and 30 mg/kg dose groups, and there was no significant difference between the 35 mg/kg and 30 mg/kg dose groups. difference. The number of TH-positive neurons in the substantia nigra region decreased, and the number of TH-positive nerve fibers in the striatum decreased.

　　CONCLUSION: A simple way to evaluate the effect of mouse Parkinson's disease model through behavioral data is established.