动物造模 z-bio 2023-04-03 445

　　Objective To investigate the effect of high-fat and high-sugar diet on fat distribution and blood biochemical indexes in Bama miniature pigs.

　　Methods There were 14 Bama miniature pigs and 8 in the experimental group. The type 2 diabetes susceptible miniature pigs cultivated in our laboratory were fed with high-fat and high-sugar feed for 30 weeks; 6 in the control group were fed with normal feed. After 30 weeks, the body shape coefficient, the fat content of CT scan, and the blood were collected to detect the biochemical indexes, and the differences between the experimental group and the control group were compared.

　　Results After 30 weeks of feeding with high-fat and high-sugar diet, there were significant differences in body mass, body mass index (BMI), and total fat content between the experimental group and the control group (P<0.05), and the difference in visceral fat content was extremely significant (P<0.01). ;Insulin, total cholesterol, high-density lipoprotein cholesterol, and triglyceride in blood biochemical indexes were significantly different from the control group (P<0.01), and the low-density lipoprotein cholesterol was significantly different from the control group (P<0.05). . In addition, the serum protein and creatinine of Bama miniature pigs in the experimental group were significantly different from those in the control group (P<0.05); the differences in uric acid and urea were extremely significant (P<0.01), indicating that the minipigs in the experimental group had kidney damage.

　　Conclusion High-fat and high-sugar diet can cause fat accumulation in Bama miniature pigs, especially visceral fat accumulation, and the blood biochemical indicators show hyperlipidemia with renal function damage.