动物造模,颅脑损伤后认知障碍 z-bio 2023-04-12 488

　　Objective To explore the preparation and evaluation of an animal model of cognitive impairment after traumatic brain injury.

　　Methods 60 healthy male rats, 48 of which were selected from the water maze sieve, were randomly divided into blank control, model control and model groups. Model preparation was carried out by electronic traumatic brain injury instrument, and water maze screening was used to identify rats with cognitive impairment after traumatic brain injury. Observe the vital signs, nerve function and motor function of the rats; Morris water maze test; measure the changes of MMP-2, MMP-9, SOD and MDA in serum.

　　Results Compared with the blank group: the vital signs of the model group were stable, and there were differences in various behavioral indicators (P<0.05). The contents of MMP-9 and MDA increased significantly (P<0.01), and SOD decreased significantly (P<0.01).

　　Conclusion This model preparation strategy can better simulate the cognitive impairment after traumatic brain injury, and at the same time has good stability, good repeatability, objective evaluation, and easy operation and mastery, which can be used for reference in similar researches.