动物造模,肠易激综合征内脏高敏 z-bio 2022-08-25 394

　　Objective To study different methods to make IBS visceral hypersensitivity mouse model, and to evaluate its visceral sensitivity and stability.

　　Methods SD rat pups on the second day after birth were randomly divided into mother-infant separation group (group A), unpredictable mother-infant separation group (group B) and blank control group (group C). After successful modeling, the general condition and defecation of the rats were observed, and the abdominal wall withdrawal reflex (AWR) score and visceral pain threshold were used to determine the visceral hypersensitivity and persistence of the rats. After the test, the colon tissue of the rats was collected Perform pathological testing.

　　Results There was no significant difference in body weight and colon pathological examination among the groups (P>0.05). Compared with groups A and C, the number of fecal particles and the water content of feces in group B increased; The AWR score of the rats in group B was higher and reached a peak at 9 weeks of age, while at 11 weeks of age, the AWR scores of the rats in groups A and B were lower than before; , the visceral pain thresholds were lower in groups A and B.

　　Conclusion Both mother-infant separation and unpredictable mother-infant separation can simulate the visceral hypersensitivity model of IBS, and the unpredictable mother-infant separation model is more suitable for making diarrhea-type IBS models. At the same time, the visceral hypersensitivity of IBS is time-dependent, and the stable period of the model is mostly from the end of modeling to within 9 weeks of age.