动物造模,颅脑损伤后认知障碍 z-bio 2022-08-25 381

　　Objective To explore the preparation and evaluation of an animal model of cognitive impairment after traumatic brain injury.

　　Methods Sixty healthy male rats (Sprague Dawley, SD) were selected and 48 were selected from the water maze screen and included in the experiment, and were randomly divided into blank control, model control and model groups. The model was prepared by using an electric Cortical Contusion Impactor (eCCI) to strike, and combined with water maze screening to determine the cognitive impairment rats after traumatic brain injury (TBI). Observe the vital signs, nerve function and motor function of the rats; Morris water maze test; measure the changes of MMP-2, MMP-9, SOD and MDA in serum.

　　Results Compared with the blank group: the vital signs of the model group were stable, and there were differences in various behavioral indicators (P<0.05). The contents of MMP-9 and MDA increased significantly (P<0.01), and SOD decreased significantly (P<0.01).

　　Conclusion This model preparation strategy can better simulate the cognitive impairment after traumatic brain injury, and at the same time has good stability, good repeatability, objective evaluation, and easy operation and mastery, which can be used for reference in similar researches.