动物造模,慢传输型便秘模型 z-bio 2022-08-25 293

　　Objective To compare the differences between the models induced by loperamide hydrochloride in slow transit constipation induced by loperamide hydrochloride and different doses and time, and to explore the establishment of a stable loperamide hydrochloride-induced slow transit constipation mouse model. experimental parameters.

　　Methods Healthy male C57BL/6 mice were used to prepare 0. 025, 0. 25 and 2. 5 mg/mL loperamide hydrochloride suspension by gavage, and the administration time was 9 am and 3 pm every day. , administered in two divided doses. The modelling effect was evaluated from the detection of intestinal propulsion rate and the detection of defecation volume, and the effects of different doses and different administration time on the mouse model of slow transit constipation were observed.

　　Results 1) Comparing the effect of different doses of loperamide hydrochloride in modeling, in the range of 0.5 mg/kg ~ 50 mg/kg, by comparing the intestinal propulsion rate between groups, the results showed that 50 mg/kg Compared with the blank group, the intestinal propulsion rate and the defecation volume of the mice in the kg group were significantly decreased, and the difference was statistically significant (P<0.05); (2) Compared with the four different groups at 3, 7, 10 and 14 days On the 7th day of modeling, the intestinal propulsion rate of the mice was significantly lower than that of the blank group (P<0.05); however, there was no significant change in the intestinal propulsion rate at 3, 10, and 14 days (P<0.05).>0.05).

　　Conclusion This study found that the continuous gavage of 50 mg/kg for 7 days can significantly reduce the intestinal propulsion rate and defecation volume in mice, and can create a relatively stable colonic transit disorder constipation mouse model, which is a The exploration of loperamide hydrochloride-induced slow transit constipation model provides valuable data.