动物造模,糖尿病模型 z-bio 2022-08-25 295

　　Objective To explore the preparation method of high-fat diet combined with streptozotocin-induced diabetic rat model, and to provide a stable diabetic animal model for clinical research.

　　Methods Thirty male SD rats were randomly divided into 2 groups: normal control group (NG, n = 10) fed with normal chow; type 2 diabetes group (DM, n = 20) fed with high fat diet (HFD) for 12 weeks +STZ 25 mg/kg tail vein injection. During the experiment, the body weight of the rats was detected every week, and the oral glucose tolerance test (OGTT), insulin tolerance test (ITT) and the area under the blood glucose curve were observed 1 week before STZ injection. After STZ injection, the general conditions of the rats (food intake, water intake, hair gloss, litter moisture and mental state) were observed, and blood glucose and fructamine were randomly detected.

　　Results Compared with the NG group, the body weight of the rats in the DM group increased significantly, and the difference was significant after 12 weeks of feeding (P<0.05). The blood glucose at 60, 90, and 120 minutes in the OGTT experiment was significantly higher than that in the NG group (P<0.05), and the area under the curve was significantly increased (P<0.05); in the IPITT experiment, the blood glucose in the DM group was significantly higher than that in the NG group (P<0.05). . 05), the area under the curve increased significantly (P < 0. 05). After STZ intervention, there were significant differences in food intake, water intake and body weight between the two groups (P < 0. 05). The random blood glucose and fructosamine in the DM group were significantly higher than those in the NG group (P < 0. 05).

　　Conclusions High-fat-fed rats induce insulin resistance and abnormal glucose tolerance after 12 weeks. Combined with low-dose STZ-induced type 2 diabetes rat model, the blood sugar is stable, which is an ideal animal model for the study of type 2 diabetes.