动物造模,肝癌模型 z-bio 2022-08-26 495

　　Objective: To explore the mechanism of hepatocarcinogenesis by establishing a C57BL/6 mouse model of chemically induced hepatocellular carcinoma to simulate the occurrence and development of hepatocellular carcinoma.

　　Methods: 110 C57BL/6 mice were selected and randomly divided into control group (30 mice) and model group (80 mice). The mice in the control group were not treated, and the model group was established by gavage administration of CCl4 combined with intraperitoneal injection of DEN. Liver cancer model; the body weight of the mice was continuously measured, the mice were dissected at different time points to observe the liver morphology, the levels of liver function-related enzymes in the venous blood serum, and the pathological changes of the liver tissue were observed.

　　RESULTS: Compared with the control group, the weight of the mice in the model group decreased gradually, the liver morphology was abnormal, the serum liver function-related enzymes ALT and AST increased, and there were obvious histopathological changes of liver cancer.

　　Conclusion: Diethylnitrosamine (DEN) combined with carbon tetrachloride (CCl4) can induce hepatocellular carcinoma in C57BL/6 mice, which provides a reliable animal model for studying the molecular mechanism of hepatocellular carcinoma.