动物造模,肺癌模型 z-bio 2022-08-26 330

　　Objective: To investigate the expression pattern of TET family proteins in mouse lung cancer model.

　　Methods: The mice were divided into 3 groups, 10 mice in each group. They were set as control group (Con), urethane-induced group (Ure), and hydroxyurea administration group (Ure-HU), respectively. The Ure group and Ure-HU group were given 800 mg/kg urethane solution for 10 consecutive weeks to induce the establishment of mouse lung cancer pattern. After 10 weeks, the Ure-HU group was given HU drug (500 mg/kg) for 21 consecutive days. Observe the number of morphological nodules in the lungs. The expression patterns of TETs in three groups of mice were analyzed by qPCR and Western blot.

　　RESULTS: Compared with the Con group, the Ure and Ure-HU groups had significant weight loss. Morphological observation showed that both Ure group and Ure-HU group had lung tissue lesions. qPCR results showed that in the Ure group, the expression of TETs was significantly decreased (P<0.05). After HU treatment, the expression of TET1 and TET2 increased in Ure-HU group (P < 0.05). Western blot and qPCR results were consistent.

　　Conclusion: In the urethane-induced mouse lung cancer model, the expression pattern of TETs protein is abnormal, which proves that TET1 and TET2 play an important role in the process of lung cancer.