动物造模,早发性卵巢功能不全 z-bio 2022-08-26 278

　　OBJECTIVE: To compare and analyze the differences of three commonly used modeling methods for early-onset ovarian insufficiency mouse models, and to explore the modeling methods that conform to the clinical characteristics of POI.

　　Methods: Ninety-six ICR mice with regular estrous cycles were randomly divided into 4 groups: A control group, B tripterygium wilfordii group, C vinylcyclohexene (VCD) group, and D galactose group. After adaptive feeding, different modeling interventions were given, and 8 mice in each group were randomly sacrificed on the 16th, 46th and 76th days after the intervention.

　　RESULTS: The E2 and follicle numbers of mice in each model group were significantly different from those in the control group; estrous cycle disorder was observed in each model group, but negative in the control group.

　　CONCLUSION: The intraperitoneal injection of VCD can damage the ovarian function of mice, resulting in a mouse POI model. This modeling method is simple and easy to implement and has a high success rate. It is more ideal among the three modeling methods and can be promoted.