动物造模,非人灵长类单侧脑损伤 z-bio 2022-08-26 404

　　Objective: By evaluating the functional changes of brain regions related to brain perception and motor behavioral responses, it is an important strategy for clinical translational research on major brain diseases based on non-human primates in brain science research. of great significance.

　　METHODS: Male cynomolgus monkeys aged 7-9 years were selected to construct a model of Parkinson's disease induced by injection of MPTP (1-methyl-4-phenyl-6-tetrahydropyridine) into the right internal carotid artery and ischemia caused by occlusion of the left middle cerebral artery. In a stroke model, a self-made mountain and valley ladder task device was used to evaluate the effect of unilateral brain region damage on contralateral sensory and motor function.

　　RESULTS: In normal monkeys, both left and right hands were able to achieve full scores in the mountain step task and valley step task for 10 consecutive days. After the monkey Parkinson's model was established by injecting MPTP into the right internal carotid artery, PET-CT image analysis showed that the three experimental monkeys were all The dopaminergic neurons in the right substantia nigra were damaged. In the mountain ladder task and the valley ladder task, two experimental monkeys showed significant damage to the motor function of the left hand, and the spatial recognition ability was basically normal, while one experimental monkey not only showed left-hand movement There may also be abnormal spatial recognition ability or impairment of right-hand motor ability; a model monkey with ischemic stroke caused by left middle cerebral artery occlusion was constructed by electrocoagulation. In the ischemic infarction, in the mountain ladder task and the valley ladder task, the right motor function of the experimental monkey was impaired, but the spatial recognition ability was basically normal.

　　CONCLUSION: The mountain and valley ladder task experiment can be effectively applied to the evaluation of motor perception behavior in the animal model of unilateral brain injury in experimental monkeys.