动物造模,原位癌模型 z-bio 2023-06-07 823

　　Objective: To select a human hepatoma cell line that stably express a luciferase reporter gene, and to construct an in situ tumor-bearing nude mouse model of human hepatoma cell line.

　　Methods: The fluorescence intensity expression of HCCLM3-Luc in different cell numbers was quantitatively analyzed by in vivo imager, and the luciferase activity of each cell number was detected to explore the linear correlation between the number of photons generated by HCCLM3-Luc luciferase and the number of tumor cells The HCCLM3-Luc tumor mass tissue in situ in the liver lobe and the HCCLM3-Luc cell suspension injected into the tail vein were used to construct a nude mouse model of human hepatocellular carcinoma in situ. The two methods were compared to construct a nude human hepatoma cell line in situ. Feasibility of mouse models and optimization of key factors in model construction.

　　Results: The number of HCCLM3-Luc cells was positively correlated (correlation coefficient R2=0.9989, linear equation: Y=1155.8X+1×106), and the average ROI value was about 140 photons/s per cell. Liver lobe in situ inoculation was used. In situ tumor formation rate [(82.5±7.2)%] was significantly higher than that of tail vein injection [(34.1±13.2)%].

　　Conclusion: In this study, a bioluminescent human hepatoma cell line in situ carcinoma in situ nude mouse model was constructed, and the model can use a small animal imager to monitor tumor growth in real time.