动物造模,白血病 z-bio 2022-08-29 388

　　Objective: To construct M1 leukemia model using BALB/c nude mice.

　　METHODS: Twelve 5-6 weeks old female BALB/cNude mice were randomly divided into low, medium and high dose groups and blank group, 3 mice in each group, and received tail vein injection. The M1 cell suspension in the growth phase was 1×106 cells per mouse, 5×106 cells per mouse and 8×106 cells per mouse. The general condition of the mice was observed, and the peripheral tissues were collected on the 0th, 10th, 20th, and 30th days after modeling. Blood samples were collected for routine blood test and leukocyte classification. The samples were sacrificed on the 30th day or at the time of death, and the proportion of CD33CD117 positive cells in peripheral blood and bone marrow was detected by flow cytometry, and histopathological sections were prepared.

　　Results: The mice in the model group appeared sluggish, less movement and arched back on the 10th to 15th day after inoculation. On the 30th day of the experiment, compared with the blank group, the number of peripheral blood leukocytes of the mice in the high-dose group was significantly increased (P<0.05); the proportion of leukemia cells in the peripheral blood of mice in each group was significantly higher than that in the blank group (P <0.05); the proportion of CD33+CD117+ in the bone marrow of mice in each group increased, and the high-dose group was the most significant (P<0.05); the high-dose group A small infiltrate of leukemia cells was seen in the spleen.

　　Conclusion: The acute myeloid leukemia model can be established in BALB/cNude mice after injection of 8×106 mouse leukemia cells M1 through the tail vein, which is in line with the biological characteristics of acute myeloid leukemia.