动物造模,动脉粥样硬化模型 z-bio 2022-08-29 275

　　Objective: To investigate the phenotypic differences and pathological mechanisms of insulin-resistant atherosclerotic models in white-haired black-eyed (WHBE) rabbits and Japanese white-eared white (JW) rabbits.

　　Methods: Twelve WHBE rabbits and 12 JW rabbits were selected and divided into normal control group (NC) and high-fat and high-sugar diet (HF) group, 6 in each group. IR-AS model was induced by HF diet for 12 weeks. End of modeling After that, blood was collected to measure blood lipid, superoxide dismutase (SOD) and malondialdehyde (MDA) levels; glucose tolerance test was performed to calculate the area under the curve of blood glucose and insulin; liver microsomal triglyceride transfer protein (MTTP), The expression of nuclear factor E2 (Nrf2) and SOD1 genes, and the pathological changes of HE staining in fat and aortic vessels, as well as the expression of vascular CD68 were observed.

　　RESULTS: Compared with the NC group, the HF group had significantly higher obesity, elevated blood lipids, glucose intolerance, hyperinsulinemia and insulin resistance index (HOMA-IR), decreased plasma and liver SOD activity and increased MDA content. The expression of MTTP and Nrf2 genes was increased, the expression of SOD1 gene was decreased, the vascular lipid deposition and AS and the expression of vascular CD68 were significantly increased; There were significant differences in U_GLU), MDA content, fat diameter, liver SOD1 gene expression, AS lesion degree and vascular CD68 expression.

　　Conclusion: High-fat and high-sugar diet can induce the formation of IR-AS in rabbits, showing lipid metabolism disorder, inflammation and AS lesions. However, the degree of lesions in WHBE rabbits is significantly more serious than that in JW rabbits, which may be related to the lipid metabolism of these two strains of rabbits. related to oxidative stress.