动物造模,深静脉血栓模型 z-bio 2022-08-29 382

　　Objective: To observe the expression and significance of thrombomodulin (TM) in the natural evolution process of deep vein thrombosis (DVT) formation and regression in rats.

　　Methods: SD rats were randomly divided into a model group (n=60) and a control group (n=6). The model group used the "stenosis method" to establish the DVT model, and the control group used the "sham operation method". Rats were sacrificed at 10 time points, 1h, 4h, 6h, 12h, 24h, 3d, 7d, 14d, 21d, and 28d. The thrombus weight/length ratio, soluble TM concentration in plasma, TMmRNA in thrombus tissue and inferior vena cava endothelium were detected. expression.

　　Results: 1) The thrombus weight/length ratio was lower in the early stage of modeling (1, 4, 6, and 12 h), and there was no significant difference among the four groups (P>0.05). A higher level (P>0.05); it began to decrease at 14d after modeling, but it was not significantly different from the results at 21 and 28d (P>0.05). (2) The ELISA results showed that the soluble TM in plasma was higher than that of the control group. The difference was significant (P<0.01), and it gradually increased over time. (3) Real-time fluorescence quantitative PCR results showed that the expression of TMmRNA in thrombus gradually increased over time. The expression of TMmRNA in vascular endothelium was at 1 Compared with the control group at ､4, 6, 12h (P<0.01); at 24h, 3d, and 7d, it was lower than the control group (P<0.01); compared="" with="" the="" control="" group="" at="" there="" was="" no="" significant="" difference="" p="">0.05). (4) Thrombus weight/length ratio was negatively correlated with endothelial TMmRNA (r=-0.92, P<0.01); while thrombus TMmRNA was positively correlated with plasma soluble TM (r=0.96, P<0.01).

　　Conclusion: The expression changes of endothelial TMmRNA can reflect the progression of DVT and have a good prognostic effect.