动物造模,实验性自身免疫性重症肌无力 z-bio 2023-02-23 365

　　Objective: To establish an experimental autoimmune myasthenia gravis animal model that meets international preclinical experimental standards.

　　METHODS: Following the methods reported in the literature and improved, the pure acetylcholine receptor (AchR) protein was extracted from the electric organ of electric rays, and the protein was qualitatively identified by SDS gel electrophoresis and quantified by BCA method. C57BL/6 mice were immunized three times (on the 1st day, the 30th day and the 60th day respectively), and the clinical score, body weight, serum AchR antibody content, neostigmine test and electromyography of EAMG mice were performed. comprehensive evaluation.

　　Results: Compared with the adjuvant group, the EAMG model group had onset from the third week, and the average clinical score increased significantly (P<0.01); the weight of the onset mice decreased significantly (P<0.01); the neostigmine test was positive; serum AchR The antibody content was significantly increased (P<0.01); EMG repeated electrical stimulation test was positive.

　　CONCLUSION: The extraction and purification of AchR protein from the electrical organs of the electric ray of the black spot electric rays successfully induced the C57BL/6EAMG mouse model, which created a good condition for further research on myasthenia gravis.