动物实验,凝血四项 z-bo 2020-08-20 477

　　Objective: To explore the best anticoagulation rate in rats and analyze the effect of insufficient blood volume of the specimen on the results of four coagulation tests.

　　Method: 60 rats were divided into two groups, and blood was collected from the abdominal aorta on an empty stomach according to the vacuum blood collection method for 12 hours. The first group of 20 animals were used to measure whole blood cells. The automatic hemocytometer can detect hematocrit (HCT) and platelet count (platelets, PLT). The second group of 40 rats, the anticoagulation ratio of each mouse [the ratio of sodium citrate anticoagulant to whole blood (V:V)] is 1:9 (control group) and 1:5 (experimental group) Centrifugation was performed at 1:8 (experimental group) and 1:7 (experimental group) to obtain platelet-poor plasma. The automatic coagulation analyzer can detect four coagulation items: prothrombin time (PT), activated partial coagulation platinum time (APTT), thrombin time (TT), fibrinogen (FIB).

　　Results: SD rats HCT (%) is 41.7±2.9, PLT (×109/L) is 1114±173. As the proportion of anticoagulant increases, PT, APTT, TT increase, and FIB decreases. Compared with the control group: 1:8 experimental group, the difference is not statistically significant. In the 1:7 experimental group, except for the statistically significant difference in TT, the differences in the other three indicators were not statistically significant. In the 1:5 experimental group, the difference was statistically significant.

　　Conclusion: The results of the four coagulation items are affected by the anticoagulation rate. 1:9 is the best anticoagulant in rats, and 1:8 is acceptable. Rats have unique physiological characteristics. It provides basic scientific data and basic theoretical basis for rat related research.