动物造模 z-bio 2022-08-29 272

　　Objective: To explore the feasibility of establishing an animal model of tree shrew norovirus infection by gavage challenge.

　　METHODS: Two groups of 6-month-old tree shrew were gavaged with fecal suspension of GII.4 HuNoVs2010 and Sydney strains respectively, and the control group was given the same volume of PBS (5 animals in each group), and the signs and diarrhea symptoms were observed for 7 days after challenge. The feces were collected every 24 hours to detect HuNoVs RNA by RT-PCR, the small intestine tissue was collected after the tree shrew was killed, the pathological changes were observed by HE staining and the virus antigen was detected by immunohistochemistry. .

　　RESULTS: Tree shrew had no obvious infection symptoms and diarrhea after gavage challenge with HuNoVs. No HuNoVsRNA was detected in fecal samples, no HuNoVs antigen was detected in small intestinal tissue, and the pathological changes were not obvious. The HBGAs antigen in tree shrew saliva was negative.

　　Conclusion: The reason why tree shrew is not susceptible to HuNoVs may be related to the fact that it does not secrete and express HBGAs and the general breeding conditions. The small animal model of HuNoVs infection in tree shrew still needs to be further explored.