动物造模,子宫内膜异位症模型 z-bio 2022-08-29 410

　　Objective: To investigate the effect of Wenjing Zhitong Recipe on the expression of AKT/mTOR pathway-related molecules in endometriosis model rats.

　　Methods: Seventy-five mature female Wistar rats that were successfully modeled were randomly divided into 5 groups, and the sham operation group was divided into 6 groups, with 15 rats in each group. Gestrigenone) capsules and intragastric administration of normal saline. Real-time PCR and immunohistochemical methods were used to detect the expression of AKT and mTOR mRNA and protein in ectopic and eutopic intima. ELISA method was used to detect vascular endothelial growth factor (vascular endothelial growth factor) in serum. endothelial growth factor, VEGF) content.

　　Results: The mRNA and protein contents of AKT and mTOR in eutopic and ectopic endometrium of endometriosis model rats were significantly higher than those in the normal control group (P<0.01), the high-dose Chinese medicine group and western medicine (gestrinone) The expression levels of AKT and mTOR mRNA in eutopic and ectopic endometrium in the EMS group were significantly lower than those in the model group (P<0.05), and the serum VEGF level in the EMS model group was higher than that in the normal control group (P<0.01). The serum VEGF levels in the high-dose, low-dose and western medicine groups were significantly lower than those in the model group (P<0.01); the middle-dose group of traditional Chinese medicine was also lower than the model group (P<0.05).

　　Conclusion: The occurrence of endometriosis is related to the AKT/mTOR signaling pathway and the expression level of VEGF. Wenjing Zhitong Fang can effectively inhibit the AKT/mTOR signaling pathway and reduce the expression of serum VEGF, thereby inhibiting the adhesion of ectopic endometrium Invasion, angiogenesis.