动物造模,疾病动物模型 z-bio 2022-08-31 375

　　Objective: To establish a green fluorescent protein (GFP)/luciferase (Luc) double-labeled human esophageal cancer ECA109 cell line (ECA109-LucGFP), and use a non-invasive biological imaging method to monitor the esophageal cancer nude mouse tumor model.

　　Methods: MTT, flow cytometry, and scratch assay were used to detect the difference in biological characteristics between ECA109-Luc-GFP cell line and normal ECA109 cells. The tumor was traced by in vivo imaging system.

　　RESULTS: Compared with normal ECA109, ECA109-Luc-GFP cells had no significant difference in cell viability, proliferation and migration ability (P>0.05). The tumor growth, metastasis and other biology were observed by small animal in vivo imaging technology. characteristic.

　　Conclusion: The GFP/Luc double-labeled human esophageal cancer ECA109 cell line (ECA109-Luc-GFP) was successfully constructed, and the growth and metastasis of the esophageal cancer animal model were observed in vivo by the application of small animal in vivo imaging technology.