动物造模,不孕症大鼠模型,疾病动物造模 z-bio 2022-09-01 322

　　Objective: To establish a rat model of acute and chronic salpingitis infertility by using Staphylococcus aureus.

　　Methods: 4×109/mL Staphylococcus aureus suspension was prepared. 108 rats were randomly divided into normal group, model group and sham operation group, 36 rats in each group. In the operation group, normal saline was injected through the fallopian tube; on the 7th, 14th, 21st, and 28th days after the operation, the morphological changes of the fallopian tubes were observed by naked eyes, and Staphylococcus aureus culture and pathological examination were performed.

　　RESULTS: Injecting Staphylococcus aureus through the fallopian tube could form an acute salpingitis model on the 7th day after surgery, a chronic salpingitis model on the 21st day, and a salpingitis infertility model on the 28th day.

　　Conclusion: Using Staphylococcus aureus at a concentration of 4×109/mL and injecting it into the fallopian tube of rats can successfully establish a rat model of acute salpingitis and chronic salpingitis infertility.