动物造模,高血压模型,疾病动物造模 z-bio 2022-09-01 202

　　OBJECTIVE: To establish a hypertensive model of Bama miniature pigs with a high-fat and high-salt diet, and to explore its possible pathogenesis.

　　Methods: A total of 18 male Bama miniature pigs were randomly divided into 3 groups: normal control (NC) group, high-fat (HF) group and high-fat and high-salt (HFHS) group, 6 in each group. The NC group was fed with normal feed. , HF group and HFHS group were fed high-fat diet and high-fat and high-salt diet, respectively, for 24 consecutive weeks. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) of minipigs were measured at 8 weeks, 16 weeks and 24 weeks after modeling. , blood glucose, blood lipid, liver and kidney function, and plasma endothelin 1 (ET-1), renin (Renin), angiotensin II (AngII), aquaporin-2 (AQP-2) were measured at 24 weeks after modeling. , vasopressin (AVP) and vascular endothelial growth factor (VEGF) and other indicators, and liver and kidney were taken for histopathological observation.

　　Results: Compared with the NC group, the SBP and DBP of the HF group and the HFHS group increased significantly after 8 weeks of modeling, and showed a continuous upward trend, and the HFHS group was higher than the HF group; at the same time, the HF group and the HFHS group after 24 weeks of modeling The body weight and liver and kidney indices of the minipigs in the HFHS group were significantly increased (P<0.05), and the plasma levels of TC, CREA and ET-1 were also significantly increased (P<0.05, P<0.01); while the BUN level in the HFHS group was significantly decreased (P<0.05), but the contents of renin, Ang-II, AQP-2, and AVP were significantly increased (P<0.05, P<0.01). Oil red "O" staining showed that the liver and kidney of HF group and HFHS group Lipid deposition occurs, and pathological changes such as renal arteriolar wall thickening occur.

　　Conclusion: High-fat and high-salt diet induced for 8 weeks can establish a model of hypertension in miniature pigs, and its pathogenesis may be related to the activation of RAS system and AVP-AQP-2 by affecting renal function.