动物造模,高尿酸血症性肾损害,疾病动物造模 z-bio 2022-09-02 373

　　Objective: To establish a reasonable and stable mouse model of hyperuricemia nephropathy, and to provide a pathological model for screening and researching drugs for the treatment of hyperuricemia nephropathy.

　　METHODS: Five modeling agents, potassium oxonate, hypoxanthine, adenine, ethambutol and yeast paste, were used alone, two drugs or three drugs in combination, and different modeling time, modeling dosage and modeling were observed. Serum uric acid, blood urea nitrogen and creatinine levels, changes in liver xanthine oxidase (XOD) and adenosine deaminase (ADA) activities in a mouse model of hyperuricemia with renal damage established by The pathological changes of kidneys and the changes of body weight in each group.

　　RESULTS: Compared with the normal group, the serum uric acid level and blood urea nitrogen of the mice in the hypoxanthine and potassium oxonate combination group were significantly increased (P<0.01). Salt crystals can be seen; the serum uric acid level and blood urea nitrogen of the mice in the 7-day combination group of hypoxanthine, ethambutol and potassium oxonate were significantly increased (P<0.01), and the liver XOD activity was significantly decreased (P<0.05) , eosinophilic insoluble protein can be seen in the proximal convoluted tubule of the kidney; compared with the normal group, the serum uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid, uric acid and Blood urea nitrogen and creatinine values were significantly increased (P<0.01). In the combined use group of yeast extract and potassium oxonate, renal tubular epithelial cells were shed, eosinophilic insoluble protein, yeast extract, adenine and oxygen were found in the proximal convoluted tubules of the kidney. Salt crystals can be seen in the renal medulla of the mice in the potassium oxonate combination group. The weight of the mice in the yeast paste and potassium oxonate combination group increased faster than that in the yeast paste, adenine and potassium oxonate combination group, and there was a difference between the two groups. Significant (P<0.05).

　　Conclusion: Compared with other modeling methods, the hyperuricemia kidney injury mouse model established by the combination of yeast paste and potassium oxonate is more stable and has no significant effect on the body weight of mice. At the same time, this modeling method is more in line with clinical practice. Therefore, it is more appropriate to use yeast paste and potassium oxonate combined for 14 days to establish a mouse model of hyperuricemia with renal damage.