动物造模,胃炎癌转化模型 z-bio 2022-09-02 292

　　OBJECTIVE: To construct a rat model of gastritis-carcinoma transformation, and to explore new serological indicators for early gastric cancer.

　　Methods: N-methyl-N'-nitro-N'-nitrosoguanidine drinking water (MNNG) combined with high-salt diet was used to induce a rat model of gastritis-cancer transformation. MRI and histopathological observation The deterioration process of rat stomach. qRT-PCR was used to detect the expression levels of serum miR-17-5p and miR-374-5p in rats at different time points (0, 1, 24, 36, and 48 weeks after modeling).

　　Results: MNNG drinking water combined with high-salt diet feeding successfully induced gastritis-carcinoma transformation in rats, which experienced atrophic gastritis (24th week of modeling) and dysplasia (48th week of modeling). The serum levels of miR-17-5p and The expression level of miR-374-5p was positively correlated with the degree of gastric deterioration in rats. Compared with the normal control group, the expression level of miR-17-5p increased about 8 times at 36 and 48 weeks respectively (t=4.1 P< 0.001) and 10-fold (t=5.3 P<0.001); at week 48, the expression level of miR-374-5p increased about 6-fold (t=3.6 0.001<P<0.002).

　　Conclusion: The rat gastritis-cancer transformation model constructed by MNNG drinking water combined with high-salt diet provides a good animal model for studying the pathogenesis of gastric cancer. Serum miR-17-5p and miR-374-5p are potential early non-invasive diagnostic indicators of gastric cancer.