动物造模 z-bio 2022-09-02 388

　　The common cotton-eared marmoset is a small non-human primate experimental animal. It has the advantages of low feeding cost, high reproductive efficiency, and short sexual maturity. It is very suitable for assisted reproductive technology and genetic modification model research.

　　The artificial assisted reproductive technology of marmosets mainly includes: semen collection, superovulation, oocyte collection, in vitro maturation, in vitro fertilization, embryo culture and transplantation. At present, the research of various technologies is relatively comprehensive, but the details still need to be improved.

　　With the rise of gene editing technology, research on marmoset gene modification based on artificial assisted reproductive technology has gradually become a hot topic. In 2009, Japan successfully carried out research on transgenic marmosets for the first time. So far, there are no reports on genetically modified marmosets in China. This article reviews recent advances in marmoset artificial assisted reproductive technology and research progress on marmoset gene modification.