动物造模,阿尔茨海默病 z-bio 2022-09-05 430

　　Objective The purpose of this study is to explore the establishment of a reliable disease-syndrome combination animal model of Alzheimer's disease and phlegm-stasis syndrome, to provide a corresponding animal model for the prevention and treatment of AD with traditional Chinese medicine, and to establish a disease-syndrome combination animal model. Provide reference.

　　Methods The APP/PS1 double transgenic mice were used as AD model animals, and the pathological state of "blood stasis" was simulated by ice water bath; the pathological state of "phlegm" was simulated by feeding with high-fat diet; the two were combined to simulate "phlegm and blood stasis". " pathological state. Different groups of mice were given different treatments to establish AD-disease model group, AD-phlegm syndrome group, AD-stasis syndrome group, and AD-phlegm-stasis syndrome group. Syngeneic non-transgenic C57BL/6J mice served as controls. After 14 days of modeling, the AD-like behavioral changes, the objective changes of tongue image, the changes of blood rheology and blood lipids, and the differences in the content of related proteins in hippocampus were detected in different groups of mice.

　　Results After 14 days of modeling, compared with the control group, there were significant changes in the behavior of mice in AD groups, and the content of related proteins increased. Compared with the AD-model group, the tongue color of the mice in AD-pathological state groups was darker and red, and the blood rheological indexes and blood lipid indexes of the AD-phlegm syndrome group were all increased, and the AD-phlegm-stasis syndrome group increased. All related indicators increased significantly.

　　CONCLUSION: Treating APP/PS1 double transgenic mice with ice-water bath combined with high-fat diet for 14 days can successfully establish an animal model of AD-phlegm and blood stasis combined syndrome. This method has a high modeling rate and is consistent with clinical symptoms. It can provide a stable animal experimental carrier for subsequent related research.